Plasmin-mediated HK degradation. Human pooled plasma was incubated for 240 min (if not otherwise indicated) at 37 with pSK, plasmin, kaolin, or bacterial supernatants from THB overnight cultures of M49 wild type (M49-WT) or the ska mutant strain ( ska) and analyzed for 120-kDa single-chain HK by immunoblotting. (A) pSK, plasmin, or kaolin was incubated in normal plasma, and plasma treated with bu

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